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insertion site in Chinese

Pronunciation:
How to pronounce "insertion site""insertion site" in a sentence

Translationmobile phoneMobile

  • 插入位點(diǎn),克隆位點(diǎn)

Examples

  • The insertion sites of tn5gusa5 were located by methods of tail - pcr and sequencing . for these 53 protease mutants , the insertion sites were distributed in 14 orfs
    運(yùn)用tail - por和測序的方法定位了轉(zhuǎn)座子tn5gusa5的插入位置，經(jīng)分析共插入14個(gè)orf中。
  • After amplifying genomic sequences flanked with tn5gusa5 by tail pcr and dna sequencing , then doing blast with genomic sequence of xcc 8004 , insertion sites of 10981 mutants were located precisely in xcc 8004 genome
    經(jīng)tail - pcr擴(kuò)增tn5gusa5旁側(cè)序列并測序，經(jīng)與xcc8004基因組序列比對后獲得精確定位的tn5gusa5插入突變體10981個(gè)。
  • The technique " improed coordinated timing of mechanical actiation of papillary muscle insertion sites and the remote decrease of functional mitral regurgitation secondary to left entricular reerse remodeling , " the authors write
    作者指出，這一技術(shù)提高了乳頭肌插入部機(jī)械出發(fā)和遠(yuǎn)隔部位二尖瓣重構(gòu)導(dǎo)致的二尖瓣功能降低性返流之間的協(xié)同考慮。
  • Based on the blast analysis and other studies , osddl mutant was a multi - copy - insertion mutant , and one of the insertion sites was in an nptii like transposase gene , whereas osdd2 , a single - copy - insertion mutant was disrupted at a gene encoded wrky transcript factor
    Osdd1突變體可能是多拷貝插入，其中一個(gè)插入到nptii轉(zhuǎn)座酶類似基因。 osdd2突變體為單拷貝、插入到一個(gè)wrky類轉(zhuǎn)錄因子基因5翻譯起始區(qū)附近。
  • The results showed that the f fragment , 728bp in length , could be a new gene with a little homology to the genes coding for polyketide synthetase or fatty - acid synthetase and the b fragment , about 4kb in length , is inferred to have repeat sequences around tn5 insertion site , in which there is homology to the wa 314 right arm of the high - pathogeniciry island of yersinia enterocolitica . to reveal any pathogenicity of enterobacter cloacae b8 and its mutated strains b8b and b8f to animals , the experiment with mice was carried out
    結(jié)果顯示， f片段長度為728bp ，與現(xiàn)有生物數(shù)據(jù)庫的blast比較分析，發(fā)現(xiàn)該序列僅有局部短于1oobp的區(qū)域與polyketide合成酶基因或與脂肪酸合成酶基因有低的同源性，推測為一新基因； b片段長約4kb ，序列拼接結(jié)果推測靠近tn5插入位點(diǎn)部位有重復(fù)序列，對b片段tn5遠(yuǎn)端的部分序列進(jìn)行blast比較，發(fā)現(xiàn)它與小腸結(jié)腸炎耶爾森氏菌的強(qiáng)毒力島有一定的同源性。
  • And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed . the genomic dna of b8 was isolated , digested with bamh i , and ligated to the adapter . using the two pairs of the primers , two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully . lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f , the other is part of the 728 bp of f fragment . this result makes it possible to continue to carry out chromosome walking , to clone and sequence the whole genes of b fragment and f fragment , and to reveal the antagonistic molecular mechanism of b8
    試驗(yàn)研究設(shè)計(jì)并合成了由40和44個(gè)堿基的寡聚脫氧核苷酸組成的染色體爬行接頭，在接頭序列和測定的f片段近tn5的序列上，設(shè)計(jì)了2對染色體爬行用的pcr引物，從b8菌株中提取基因組dna ， bamhi酶切，與染色體爬行接頭連接，依次用2對引物進(jìn)行pcr ，擴(kuò)增出239bp產(chǎn)物，經(jīng)克隆、測序，發(fā)現(xiàn)其中18bp為擴(kuò)增的相應(yīng)于f片段在b8f菌株tn5插入位點(diǎn)對面的序列，其余則為f片段728bp序列的一部分，為進(jìn)一步進(jìn)行染色體爬行，克隆和測定整個(gè)b和f基因，揭示陽菌株的拮抗分子機(jī)制提供了技術(shù)資料貯備。
  • In addition , molecular experiments indicated that resistance was not relevant to copy number or to insertion sites , but instead to the repeated gfp structure and to methylation of genomic dna . pvx vectors were constructed that contained the s6 gene of rbsdv vi and the 14 kd fragment of bnyvv rna2
    分子檢測結(jié)果顯示， gfp3植株中普遍存在gfp基因的沉默現(xiàn)象，且gfpdna的甲基化程度明顯高于gfp1植株，說明這些植株對于病毒的抗性與目的基因的插入位點(diǎn)及拷貝數(shù)和無關(guān)，但與串聯(lián)重復(fù)的gfp基因結(jié)構(gòu)形式密切相關(guān)。

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